Evaluation Deoxyribonucleic acid (DNA) fragmentation of local Indonesian cattle frozen sperm using Halomax method

Abstract. Prabowo TA, Bintara S, Yusiati LM, Sitaresmi PI, Widayati. 2023. Evaluation Deoxyribonucleic acid (DNA) fragmentation of local Indonesian cattle frozen sperm using Halomax method. Biodiversitas 24: 2225-2230. is a molecular chain containing genetic material that determines the quality each individual produced. DNA damage in can affect fertility and miscarriage embryos. There no simple research on (Bos indicus Linnaeus 1758); therefore, infertility cases Indonesia are still high, one which be attributed to damage. This study aimed evaluate semen suggested SDF (Sperm Fragmentation) inclusion screening as main requirement for semen. The materials used this were 54 straws from nine males three different types cattle, including Bali, Madura, Aceh. Each type consisted bulls, with six replicates. Sperm-Bos-Halomax was examine Data analyzed one-way analysis variance (ANOVA). Aceh had highest post average compared Bali Madura at 63.33±2.43%. Abnormality Balinese lowest 8.83±1.04%. Sperm-Bos-Halomax® method showed 1.72±0.62% Aceh, 1.67±0.66% cattle. 1.67±0.70%. good, but it necessary determine standard